FIGURE

Fig. 6

ID

ZDB-FIG-120525-58

Publication

Zhang et al., 2012 - beta-Catenin 1 and beta-catenin 2 play similar and distinct roles in left-right asymmetric development of zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

ctnnb1 and ctnnb2 in DFCs are required for KV and cilia formation. Embryos were injected with individual or mixed morpholinos at the 512-cell stage and visualized after immunostaining at the 10-somite stage. (A-E) KV cilia were visualized by anti-acetylated-tubulin antibody. Morphants had fewer and shorter cilia. Scale bar: 20 μm. (A′-E′) KV lumen cells labeled by anti-ZO-1 antibody. The embryos were identical to those in A-E. (F-J) The KV fluid flow was monitored by observing movements of injected fluorescent beads. Each colored line represented the kinetic track of a single bead and the arrowhead indicates the moving direction. The moving paths in morphants were disordered. (K,L) Quantification of cilia number (K) and length (L). Error bars indicate ±s.e.m.; n, analyzed embryo number. Statistical significance: ns, P>0.05; ^**P<0.01. Student’s t-test was used.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development