|
Fig. 6
ctnnb1 and ctnnb2 in DFCs are required for KV and cilia formation. Embryos were injected with individual or mixed morpholinos at the 512-cell stage and visualized after immunostaining at the 10-somite stage. (A-E) KV cilia were visualized by anti-acetylated-tubulin antibody. Morphants had fewer and shorter cilia. Scale bar: 20 μm. (A′-E′) KV lumen cells labeled by anti-ZO-1 antibody. The embryos were identical to those in A-E. (F-J) The KV fluid flow was monitored by observing movements of injected fluorescent beads. Each colored line represented the kinetic track of a single bead and the arrowhead indicates the moving direction. The moving paths in morphants were disordered. (K,L) Quantification of cilia number (K) and length (L). Error bars indicate ±s.e.m.; n, analyzed embryo number. Statistical significance: ns, P>0.05; **P<0.01. Student’s t-test was used.