FIGURE

Fig. 2

ID

ZDB-FIG-120522-16

Publication

Das et al., 2012 - Bmps and id2a act upstream of twist1 to restrict ectomesenchyme potential of the cranial neural crest

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Gene expression profiling in twist1a/1b-MO embryos.

(A,B) sox10:GFP-positive and -negative cells were isolated from un-injected or twist1a/1b-MO embryos at 18 hpf by FACS. (C) Fold changes of the GFP+/GFP ratios between twist1a/1b-MO and un-injected controls show the top 25 up-regulated (blue) and down-regulated (red) genes after Twist1 depletion. Color codes indicate where genes are expressed based on the published literature (see Table S1 for references). (D–O) Confocal projections of fluorescent in situ hybridizations show expanded expression of gch2 and nr4a2b at 18 hpf, ectopic arch expression (arrows) of sox10 and foxd3 at 24 hpf, but no change in sox9b and tfap2a expression at 24 hpf in twist1a/1b-MO versus un-injected controls. Scale bar = 50 μm.

Expression Data

Genes:	foxd3 gch2 GFP nr4a2b sox9b sox10 tfap2a
Fish:	WT el8Tg WT + MO1-twist1a + MO3-twist1b
Knockdown Reagents:	MO1-twist1a MO3-twist1b
Anatomical Terms:	cranial neural crest pharyngeal arch
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.