FIGURE

Fig. 3

ID

ZDB-FIG-120522-17

Publication

Das et al., 2012 - Bmps and id2a act upstream of twist1 to restrict ectomesenchyme potential of the cranial neural crest

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Fig. 3

Fgf signaling depends on Twist1 and regulates a subset of ectomesenchyme gene expression.

(A,B) In situs at 18 hpf show that expression of the Fgf target gene pea3 is reduced in the arches (numbered) of twist1a/1b-MO embryos (n = 8/8) compared to un-injected controls (n = 0/12). (C–L) sox10:Gal4VP16; UAS:dnFgfr1a embryos show ectopic arch expression of sox10 at 18 hpf (n = 8/8) versus controls (n = 0/8), reduction of dlx2a at 24 hpf (n = 3/3) versus controls (n = 0/3), but no change in fli1a at 24 hpf (n = 0/7) versus controls (n = 0/5). twist1a expression was unchanged at 24 hpf in dnFgfr1a embryos (n = 10) compared to controls (n = 7), as was twist1b expression in dnFgfr1a embryos (n = 8) compared to controls (n = 8). Black arrows indicate the second arch, white arrowheads the ear, and red arrowheads the vasculature. Scale bar = 50 μm.

Expression Data

Genes:	dlx2a etv4 fli1 sox10 twist1a twist1b
Fish:	WT el159Tg el159Tg; el28Tg WT + MO1-twist1a + MO3-twist1b
Knockdown Reagents:	MO1-twist1a MO3-twist1b
Anatomical Terms:	otic vesicle pharyngeal arch pharyngeal arch 1 pharyngeal arch 2 pharyngeal arch 3 pharyngeal arch 4 vasculature
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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