FIGURE

Fig. 1

ID

ZDB-FIG-120508-27

Publication

English et al., 2012 - Incomplete splicing, cell division defects, and hematopoietic blockage in dhx8 mutant zebrafish

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Fig. 1

mmy mutants have severe defects in primitive hematopoiesis. A: Whole-mount in situ hybridization of myeloid markers (l-pln, lysc, and mpo) at 24 hpf. B: Expression of erythroid markers αe1 and αe2 globins, band3 and gata1 at 21 somite. C: Stem cell markers cbfb and scl are also down-regulated in mmy mutants. D: Acridine orange staining showing extensive apoptosis in mmy mutants through the entire embryos. For all probes tested, we typically used 30–40 embryos per hybridization. As such, 25% (8–10 embryos) will be mmy.

Expression Data

Genes:	cbfb gata1a hbae1.1 hbae3 lcp1 lyz mpx slc4a1a tal1
Fish:	EKW dhx8^hg9/hg9; y1Tg (EKW)
Anatomical Terms:	erythroid lineage cell intermediate cell mass of mesoderm myeloid cell
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dhx8^hg9/hg9; y1Tg (EKW)
Observed In:	apoptotic process blood cell hemopoiesis post-vent region whole organism
Stage Range:	10-13 somites to Prim-15

Phenotype Detail

Acknowledgments

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