olig2 MO does not disrupt lateral line migration. All panels show dorsal views of whole embryos, anterior to the left. Elavl labeling is shown in red and Tg(isl1:GFP)rw0 reporter expression is green. A–C: 28-hpf embryos. D–F: 33-hpf embryos. G–I: 50-hpf embryos. A–F show projections of z-stack images in both red and green channels. The red channel in G–I is a single z-plane, focused on the lateral line, whereas the green channel is a z-stack image projection of the green channel. Circles mark the posterior lateral line primordia. Arrows indicate lagging facial motor neurons in olig2 MO-injected embryos. Scale bar = 30 μm.
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