FIGURE

Fig. 1

ID

ZDB-FIG-120301-39

Publication

Wan et al., 2012 - HB-EGF Is Necessary and Sufficient for Müller Glia Dedifferentiation and Retina Regeneration

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Fig. 1

Injury-Dependent hb-egf_a Induction Is Necessary for MG Dedifferentiation and Proliferation (A) RT-PCR showing the temporal expression pattern of genes encoding EGFR ligands in the uninjured and injured retina. (B) In situ hybridization and BrdU immunofluorescence show that hb-egf_a is induced in proliferating progenitors in the injured retina (arrows) (_^* marks the injury site). (C) MO-mediated HB-EGF_a knockdown reduces the number of proliferating MG-derived progenitors in the injured retina at 4 dpi. Arrows identify proliferating cells in the control MO-treated retina, whereas arrowheads identify proliferating cells in the HB-EGF_a knockdown retina (note that these latter cells lack lissamine-labeled MO). (D) Quantification of (C) at different hb-egf_a MO concentrations. ^*p < 0.01. Error bars represent SD. Scale bars, 50 μm. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.

Expression Data

Genes:	btc hbegfa tgfa
Fish:	WT
Condition:	physical alteration: anatomical structure
Anatomical Terms:	Muller cell retina
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-hbegfa
Condition:	physical alteration: anatomical structure
Knockdown Reagent:	MO1-hbegfa
Observed In:	cellular response to mechanical stimulus Muller cell retina development in camera-type eye sensory epithelium regeneration
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 22(2), Wan, J., Ramachandran, R., and Goldman, D., HB-EGF Is Necessary and Sufficient for Müller Glia Dedifferentiation and Retina Regeneration, 334-347, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell