Fig. 1

Wnt/β-catenin signaling regulates LR asymmetry KV cell-autonomously. (A-C) fzd10 expression pattern. Shown are a lateral view of 80% epiboly (A), and lateral (B) and dorsal (C) views of the tail bud region of 10-somite staged zebrafish embryos with anterior to the left. Arrow, DFCs; arrowhead, KV. (D) Western blot showing diminished Fzd10 level in fzd10 morphants. Actin was used as a loading control. (E-H) fzd10 transduces Wnt/β-catenin signaling. DFC-specific injection of fzd10 MO depleted sp5l expression from DFCs (F). Knockdown of Fzd10 resulted in downregulation of axin2 expression (H). Shown are lateral views of 80% epiboly (E,F) and 10-somite (G,H) staged embryos. Arrow indicates DFCs. (I) Representative images of spaw expression (bracket) in DFC^{fzd10 MO} embryos at the 21-somite stage. (J) DFC/KV-specific reduction of Wnt/β-catenin signaling randomizes spaw expression. Percentages of spaw expression were determined in 21-somite staged embryos. MO (ng), amount of MO used; N, number of embryos examined. L, left-side; R, right-side; A, absence; B, bilateral. ^*P<0.01 compared with corresponding modulations in the yolk only or with uninjected controls.