FIGURE

Fig. 3

ID

ZDB-FIG-120126-70

Publication

Nguyen et al., 2012 - An inducible krasV12 transgenic zebrafish model for liver tumorigenesis and chemical drug screening

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Fig. 3

Roles of the Raf-MEK-ERK and PI3K-AKT-mTOR pathways during kras^V12 tumor progression and regression. (A) Western blots using total liver proteins from induced driver-effector fish with HL or HCC, as well as regressed liver samples, to detect total Kras (F234), mutant Kras-2B (C19) isoform, phospho-ERK1/2 (P-ERK1/2), P-AKT and P-S6 proteins. The non-induced driver, induced-driver and non-induced driver-effector having normal liver (N) served as controls.β-actin, internal control for equal loading. (B) Immunohistochemical staining showing phospho-ERK1/2 and -AKT protein as well as apoptotic cells (TUNEL) during kras^V12-dependent liver tumor progression and regression. Asterisks and dotted lines indicate the region of tumor scar.

Expression Data

Antibodies:	Ab1-kras Ab2-kras
Fish:	gz23Tg gz23Tg; gz24Tg
Condition:	chemical treatment: mifepristone
Anatomical Term:	liver
Stage Range:	Days 21-29 to Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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