FIGURE

Fig. 3

ID

ZDB-FIG-120125-14

Publication

Powell et al., 2011 - Jamb and jamc are essential for vertebrate myocyte fusion

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Fig. 3

Fast and slow-twitch muscle fibres are correctly specified in jamb^HU3319 and jamc^sa0037 embryos.

(A) Superimposed confocal microscopy images of 24 h. p. f. wild-type, jamb^HU3319, and jamc^sa0037 embryos stained with monoclonal antibody F59, which detects slow-muscle-specific myosin heavy chain (sMyHC), show normal slow muscle development in both mutants. (B) Single optical sections of 48 h. p. f. wild-type, jamb^HU3319, and jamc^sa0037 embryos stained with monoclonal antibody EB165, which detects fast-muscle-specific myosin heavy chain (fMyHC), show that the medial mononuclear fibres in both mutants have differentiated as fast-twitch muscle. Myotomes 12–13 shown, anterior left. Scale bars represent 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jam2a^{hu3319/hu3319} jam3b^sa37/sa37
Observed In:	muscle cell fate specification slow muscle cell
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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