FIGURE

Fig. 2

ID

ZDB-FIG-120125-11

Publication

Powell et al., 2011 - Jamb and jamc are essential for vertebrate myocyte fusion

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Fig. 2

jamb and jamc are essential for myocyte fusion in vivo.

(A) Schematics of Jamb and Jamc extracellular proteins. Red stars denote sites of mutation in HU3319 and sa0037 alleles. (B–C) Confocal microscopy images of fast-twitch muscle in wild-type, jamb^HU3319, and jamc^sa0037 48 h. p. f. (B) and 120 h. p. f. (C) Embryos labelled with membrane targeted RFP (mRFP, cyan; B) or phalloidin-Alexa488 (cyan; C) and DAPI (red) show overabundant, mononuclear myofibres in both mutants. (D) Confocal microscopy images of uninjected, jamb, and jamc translation-blocking morpholino-injected wild-type embryos, stained with DAPI (red) and phalloidin-Alexa488 (cyan) to stain F-actin in fast muscle fibres. The morpholino-injected embryos replicate the jam mutants′ phenotype. Myotomes 12–13 shown, anterior left. Scale bars represent 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jam2a^{hu3319/hu3319} jam3b^sa37/sa37 WT + MO1-jam2a WT + MO1-jam3b
Knockdown Reagents:	MO1-jam2a MO1-jam3b
Observed In:	fast muscle cell myoblast fusion
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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