FIGURE

Fig. 5

ID

ZDB-FIG-111102-27

Publication

Yin et al., 2011 - Mutant Prpf31 causes pre-mRNA splicing defects and rod photoreceptor cell degeneration in a zebrafish model for Retinitis pigmentosa

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Fig. 5

Accumulation of AD5 in rod cytoplasm analyzed in Tg(Rho:AD5:mCherry) larvae at different stages. (A) At 6 dpf, AD5:mCherry was exclusively localized to the nuclei of rods (arrows). (B) In contrast, AD5:mCherry was found also in the inner segments (arrowheads) of rods at 14 dpf. (C) Tg(Rho:Prpf31:mCherry) transgenic larvae at 14 dpf were used as control to show that Prpf31:mCherry is only found in rod nuclei (arrow) but not the cytoplasm. (D) In 21 dpf Tg(Rho:AD5:mCherry) larvae, AD5 protein as detected by PRPF31 antibody (green) was co-localized with mCherry (red) in both the nucleus (arrow) and rod inner segment (arrowhead). (E) In 21 dpf wild-type fish, endogenous Prpf31 protein was localized in all nuclei. Note that imaging exposure in E was extended compared to D to reveal nuclear localization of Prpf31 in all retinal cells. Shorter exposure in D indicates strong expression of transgenic AD5 under control of the rhodopsin promoter in rod photoreceptors.

Expression Data

Genes:	mCherry prpf31
Fish:	WT ns2Tg/+ ns3Tg/+
Anatomical Terms:	retinal neural layer nucleus retinal rod cell nucleus retinal rod cell photoreceptor inner segment
Stage Range:	Day 6 to Days 21-29

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ns3Tg/+
Observed In:	retinal rod cell photoreceptor inner segment
Stage Range:	Days 14-20 to Days 21-29

Phenotype Detail

Acknowledgments

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