FIGURE

Fig. 4

ID

ZDB-FIG-111102-26

Publication

Yin et al., 2011 - Mutant Prpf31 causes pre-mRNA splicing defects and rod photoreceptor cell degeneration in a zebrafish model for Retinitis pigmentosa

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Fig. 4

Subcellular localization of wild-type and mutant Prpf31 fusion proteins in rod photoreceptors. Wild-type and mutant Prpf31 fused to mCherry and driven by the rhodopsin promoter were transiently expressed in 7 dpf Tg(Rho:EGFP) larvae in which EGFP is stably expressed in rods and distributed throughout the cytoplasm. (A) Control mCherry was localized in the rod cytoplasm similar as EGFP. (B) Wild-type Prpf31:mCherry was localized in the nuclei of rods. (C) AD5:mCherry was mainly detected in the nuclei of rods similar to wild-type Prpf31. (D) In exceptional cases, AD5:mCherry was also observed in the cytoplasm of individual rods (arrows). Some of the AD5:mCherry positive rods show loss of the outer segment and disintegrated nuclei (arrow head) indicative for rod degeneration. (E) SP117:mCherry was aberrantly localized in the cytoplasm of rods rather than the nucleus. Morphology, however, is normal in SP117 expressing rods. ROS, rod out segment; RIS, rod inner segment; RCB,rod cell body. Left column shows EGFP channel, middle column shows mCherry channel and right column shows overlay together with DAPI stain.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurodegener.