FIGURE

Fig. 6

ID

ZDB-FIG-111102-15

Publication

Gore et al., 2011 - Rspo1/Wnt signaling promotes angiogenesis via Vegfc/Vegfr3

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Fig. 6

Expression of vegfc is specifically regulated by Wnt/β-catenin signaling. (A-D) In situ hybridization of the mid-trunk of 24 hpf of wild-type sibling (A,C), etsrp^y11 mutant (B) and flk^y17 mutant (D) embryos, probing for vegfc. Arrows show vegfc expression in the dorsal aorta, which is not affected in the two mutants. (E,F) RT-PCR analysis showing expression of vegfc by cultured mouse endothelial cells upon treatment with BIO (E) or Wnt3a (F). (G,H) In situ hybridization of 26 hpf rspo1 MO-injected embryos treated from 16-26 hpf with either 0.5% DMSO carrier (G) or 0.5 μM BIO in DMSO (H), probed for vegfc (arrows). (I,J) In situ hybridization of 36 hpf wild-type sibling (I) or homozygous (apc^mcr/apc^mcr) mutant (J) embryos probed for vegfc (arrows). Scale bars: 50 μm.

Expression Data

Gene:	vegfc
Fish:	WT apc^hu745/hu745 etsrp^y11/y11 kdrl^y17/y17 WT + MO3-rspo1
Condition:	chemical treatment: pharmaceutical
Knockdown Reagent:	MO3-rspo1
Anatomical Term:	dorsal aorta
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	etsrp^y11/y11 kdrl^y17/y17
Observed In:	angiogenesis axial blood vessel intersegmental vessel
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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