FIGURE

Fig. 7

ID

ZDB-FIG-111028-8

Publication

Hale et al., 2011 - Netrin Signaling Breaks the Equivalence between Two Identified Zebrafish Motoneurons Revealing a New Role of Intermediate Targets

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Netrin mRNAs are expressed in intermediate targets of the CaP axon and wildtype transcripts were knocked down with SBMOs.

A. Projected confocal stack of ntn1a and ntn1b mRNA expression in zebrafish trunk at 24 hpf; ntn1a is expressed in spinal cord (purple) and muscle pioneers (purple and *). ntn1a staining was false colored purple. ntn1b mRNA (green) is expressed in floor plate (white dots) and hypochord, located ventrally to the notochord. B. Brightfield image of lateral surface of zebrafish trunk at 24 hpf reveals ntn2 is expressed in ventral somite (white arrowheads). Asterisks denote muscle pioneers, cells that do not express ntn2. C. RT-PCR results confirming uninjected embryos have wildtype ntn1b (500 bp band) and ntn2 (300 bp band) transcript. Starred lanes show netrin transcripts are reduced or absent after injecting 10.0 ng of ntn1b SBMO or 5.0 ng of ntn2 SBMO. Multiple samples were run in the gels for ntn1b and ntn2. Lanes containing samples unrelated to the netrins were cropped from the figure. Scale bar = 20 μm.

Expression Data

Genes:	ntn1a ntn1b ntn2
Fish:	WT
Anatomical Terms:	floor plate hypochord muscle pioneer myotome ventro-lateral region somite ventral region spinal cord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One