FIGURE

Fig. 6

ID

ZDB-FIG-111026-6

Publication

Smith et al., 2011 - Bmp and Nodal Independently Regulate lefty1 Expression to Maintain Unilateral Nodal Activity during Left-Right Axis Specification in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Bmp and Nodal induce lefty1 independently.

(A–D) Tg(hsp70l:bmp2b) embryos were left at 28°C (A,C) or heat-shocked at 10 hpf to induce bmp2b expression (B,D). A subset of embryos were incubated in the presence of the Nodal inhibitor SB431542 directly after the heat-shock. Embryos were analysed by in situ hybridisation for lefty1 expression at 15-somites. (E–H) Tg(hsp70l:nog3) embryos were left at 28°C (E,G) or heat-shocked at 10 hpf to induce noggin3 expression (F,H). In a subset of embryos a bead preincubated with recombinant Nodal was placed in the ALPM. Embryos were analysed by in situ hybridisation for lefty1 expression at 18-somites. All embryos are shown as lateral views with dorsal to the right and anterior to the top. Arrows point to most anterior lefty1 expression domain. Numbers in lower right represents the number of embryos that displayed the phenotype represented in the panels.

Expression Data

Gene:	lft1
Fish:	fr13Tg fr14Tg
Condition:	heat shock
Anatomical Terms:	notochord notochord posterior region
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.