FIGURE

Fig. S1

ID
ZDB-FIG-111017-17
Publication
Lenhart et al., 2011 - Two additional midline barriers function with midline lefty1 expression to maintain asymmetric Nodal signaling during left-right axis specification in zebrafish
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Fig. S1

Characterization of the novel bmp4S355* and bmp4C365S mutant alleles. (A) Diagram of the Bmp4 protein with locations of the S355* and C365S mutations indicated. (C,E) Sequence results from PCR amplification of the bmp4 gene from cDNA obtained from wild type (wt), bmp4C365S (C) and bmp4S355* (E) mutants confirming the identity and location of genetic lesions. (B,D,F-J) Brightfield images of zebrafish at 24-36 hpf. (B) WT embryo. (D) bmp4C365S mutant with no obvious defects in the ventral fin or cloaca. (F) bmp4S355* mutant exhibiting significant loss of the ventral fin (arrow). (G) Uninjected embryo. (H) Embryo injected with 50 pg of S355* RNA exhibiting no morphological defects. (I,J) Embryos injected with 50 pg of C365S RNA displaying C2-C3 (I) and C4 (J) dorsalization. Images of WT and uninjected embryos are duplicated from Fig. 2.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
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