FIGURE

Fig. S1

ID

ZDB-FIG-111017-17

Publication

Lenhart et al., 2011 - Two additional midline barriers function with midline lefty1 expression to maintain asymmetric Nodal signaling during left-right axis specification in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Characterization of the novel bmp4^S355* and bmp4^C365S mutant alleles. (A) Diagram of the Bmp4 protein with locations of the S355* and C365S mutations indicated. (C,E) Sequence results from PCR amplification of the bmp4 gene from cDNA obtained from wild type (wt), bmp4^C365S (C) and bmp4^S355* (E) mutants confirming the identity and location of genetic lesions. (B,D,F-J) Brightfield images of zebrafish at 24-36 hpf. (B) WT embryo. (D) bmp4^C365S mutant with no obvious defects in the ventral fin or cloaca. (F) bmp4^S355* mutant exhibiting significant loss of the ventral fin (arrow). (G) Uninjected embryo. (H) Embryo injected with 50 pg of S355* RNA exhibiting no morphological defects. (I,J) Embryos injected with 50 pg of C365S RNA displaying C2-C3 (I) and C4 (J) dorsalization. Images of WT and uninjected embryos are duplicated from Fig. 2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bmp4^b1149/b1149 bmp4^b1150/b1150 bmp4^b1149/+ bmp4^b1150/+
Observed In:	cloaca development cloacal chamber ventral fin fold
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development