Fig. S1

Fig. S1 Characterization of the novel bmp4^S355* and bmp4^C365S mutant alleles. (A) Diagram of the Bmp4 protein with locations of the S355* and C365S mutations indicated. (C,E) Sequence results from PCR amplification of the bmp4 gene from cDNA obtained from wild type (wt), bmp4^C365S (C) and bmp4^S355* (E) mutants confirming the identity and location of genetic lesions. (B,D,F-J) Brightfield images of zebrafish at 24-36 hpf. (B) WT embryo. (D) bmp4^C365S mutant with no obvious defects in the ventral fin or cloaca. (F) bmp4^S355* mutant exhibiting significant loss of the ventral fin (arrow). (G) Uninjected embryo. (H) Embryo injected with 50 pg of S355* RNA exhibiting no morphological defects. (I,J) Embryos injected with 50 pg of C365S RNA displaying C2-C3 (I) and C4 (J) dorsalization. Images of WT and uninjected embryos are duplicated from Fig. 2.