FIGURE

Fig. 5

ID

ZDB-FIG-110920-7

Publication

Gao et al., 2011 - Wdr18 Is Required for Kupffer's Vesicle Formation and Regulation of Body Asymmetry in Zebrafish

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Fig. 5

Correct Ca²⁺ distribution in the KV during early somite stages requires wdr18.

(A–F) Embryos were injected at the 1-cell stage with Ca²⁺ indicator (Ci) or co-injected with wdr18 MO1. Ca²⁺ patterns at the 5- to 8-somite stage were imaged by fluorescence microscopy and the images were converted to an intensity scale (colors from red to green indicate intensity from high to low). (A, B) The autofluorescent image of an un-injected control embryo (B) with its corresponding DIC image (A). (C, D) A control embryo injected with Ca²⁺ indicator only, showing fluorescence in the KV with elevated signals on the left side. (E, F) An embryo co-injected with Ca²⁺ indicator and wdr18 MO1. The KV is smaller and the fluorescence signal is evenly distributed throughout the KV. nc: notochord. The ratio shown in the lower right corner of B, D and F indicate the number of defective embryos versus total embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-wdr18
Knockdown Reagent:	MO1-wdr18
Observed In:	Kupffer's vesicle
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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