FIGURE

Fig. 5

ID

ZDB-FIG-110907-10

Publication

Cheung et al., 2011 - Visualization, characterization and modulation of calcium signaling during the development of slow muscle cells in intact zebrafish embryos

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Fig. 5

An example of the Ca²⁺ signals generated in the trunk of a wild-type embryo at ~17.5 hpf (i.e., the 17-somite stage), as visualized by confocal microscopy using calcium green-1 dextran. (Ai-Avi) Representative (n=3) single confocal sections to show the Ca²⁺ signals generated in the dorsal half of somite 7, 8 and 9 (see the schematic, panel Ai*). The time interval between each image was ~1.2 sec. The color scale represents the level of [Ca²⁺]i, where red indicates a high level and blue indicates a low level. Ant. and Pos. are anterior and posterior, respectively. Scale bar, 50 μm. (Bi) Temporal profile of the average calcium green-1 dextran fluorescence intensity (in Arb. units) recorded in an ROI covering ~4-5 SMCs (i.e., ~800 μm²) placed in the dorsal part of somite 8 of this wild-type embryo at ~17.5 hpf (see schematic, panel Bi*). (Bii) Temporal profile of the average rhodamine B dextran fluorescence intensity (in Arb. units) recorded in the same ROI over the same time period.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.