ZFIN Image: Cheung et al., 2011, Fig. 5

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Figure Caption

Fig. 5

An example of the Ca²⁺ signals generated in the trunk of a wild-type embryo at ~17.5 hpf (i.e., the 17-somite stage), as visualized by confocal microscopy using calcium green-1 dextran. (Ai-Avi) Representative (n=3) single confocal sections to show the Ca²⁺ signals generated in the dorsal half of somite 7, 8 and 9 (see the schematic, panel Ai*). The time interval between each image was ~1.2 sec. The color scale represents the level of [Ca²⁺]i, where red indicates a high level and blue indicates a low level. Ant. and Pos. are anterior and posterior, respectively. Scale bar, 50 μm. (Bi) Temporal profile of the average calcium green-1 dextran fluorescence intensity (in Arb. units) recorded in an ROI covering ~4-5 SMCs (i.e., ~800 μm²) placed in the dorsal part of somite 8 of this wild-type embryo at ~17.5 hpf (see schematic, panel Bi*). (Bii) Temporal profile of the average rhodamine B dextran fluorescence intensity (in Arb. units) recorded in the same ROI over the same time period.

Acknowledgments

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