FIGURE

Fig. 6

ID

ZDB-FIG-110816-5

Publication

Kabashi et al., 2011 - FUS and TARDBP but Not SOD1 Interact in Genetic Models of Amyotrophic Lateral Sclerosis

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Fig. 6

Mutant SOD1 exacerbates the motor phenotype caused by mutant FUS and mutant TARDBP.

A) Motor phenotype was assessed both by time frames obtained from video recordings of the TEER as well as B) immunohistochemical labeling of the axonal projections to determine UAL in motor neurons. The analysis demonstrates that knock-down of both Tardbp and Fus (ii) does not lead to an exacerbated phenotype when compared to phenotypes generated by KD of Tardbp (i) or Fus by AMOs. Similarly, overexpression of both mutant R521H FUS and G348C TARDBP (iv) does not aggravate the phenotype observed by overexpression of mutant TARDBP (iii) or mutant FUS (Figure 2A, 2Biv). However, co-expression of mutant SOD1 and mutant FUS did exacerbate the motor phenotype caused by mutant SOD1 alone (v). Arrowheads represent length to the first axonal branching (UAL). Scale bar: 40 μm.

Expression Data

Antibody:	Ab-SV2
Fish:	WT + MO1-tardbpb WT + MO1-fus + MO1-tardbpb
Knockdown Reagents:	MO1-fus MO1-tardbpb
Anatomical Term:	motor neuron axon
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tardbpb WT + MO1-fus + MO1-tardbpb
Knockdown Reagents:	MO1-fus MO1-tardbpb
Observed In:	motor neuron axon sensory perception of touch
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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