ZFIN Image: Kabashi et al., 2011, Fig. 6

Image

Figure Caption

Fig. 6

Mutant SOD1 exacerbates the motor phenotype caused by mutant FUS and mutant TARDBP.

A) Motor phenotype was assessed both by time frames obtained from video recordings of the TEER as well as B) immunohistochemical labeling of the axonal projections to determine UAL in motor neurons. The analysis demonstrates that knock-down of both Tardbp and Fus (ii) does not lead to an exacerbated phenotype when compared to phenotypes generated by KD of Tardbp (i) or Fus by AMOs. Similarly, overexpression of both mutant R521H FUS and G348C TARDBP (iv) does not aggravate the phenotype observed by overexpression of mutant TARDBP (iii) or mutant FUS (Figure 2A, 2Biv). However, co-expression of mutant SOD1 and mutant FUS did exacerbate the motor phenotype caused by mutant SOD1 alone (v). Arrowheads represent length to the first axonal branching (UAL). Scale bar: 40 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.