FIGURE

Fig. S4

ID

ZDB-FIG-110811-1

Publication

Matsui et al., 2011 - Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis

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Fig. S4

DFC-specific knockdown of cnpy1 does not affect DFC migration towards the vegetal pole. (A-J) Time-lapse confocal imaging of DFC migration in DFC^control-MO-injected (A-E) or DFC^cnpy1-MO-injected (F-J) embryos. DFCs were labeled with SYTO17 tracer, and DFC migration was monitored every 2.5 min for 82.5 min. A, F; 0 min, B, G; 20 min, C, H; 40 min, D, I; 60 min. Although a DFC cluster (arrow in A) is found in the DFC^control-MO embryo at 0 min, sparse DFC populations (arrows in F) appear in the DFC^cnpy1-MO embryo. AP, animal pole; VP, vegetal pole. (E, J) Three cells in each embryo at 0 min are marked by red dots, and their migration is traced at 20-min intervals (indicated by color changes from red [0 min] to green [60 min]).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA