FIGURE

Fig. 2

ID

ZDB-FIG-110719-7

Publication

Li et al., 2011 - Zebrafish krüppel-like factor 4a represses intestinal cell proliferation and promotes differentiation of intestinal cell lineages

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Fig. 2

Phenotype and histological analysis of klf4a-MO1 and klf4a-MO2-injected morphants and the specificity of klf4a-MOs by Western blot analyses.

(A) Western blot analyses demonstrate that co-injection of klf4a-MO1 and klf4a-MO2 (1.5 ng each) prevents the synthesis of klf4a-T7 fusion protein. Similar α-tubulin expression levels are shown in embryos with different treatments. (B) Phenotype comparison among wild type, klf4a-5mmMO2-injected, and klf4a-MO1 and klf4a-MO2-injected embryos at 24 hpf. * indicates the enlargement of midbrain ventricle and the arrow indicates the reduced otic vesicle. (C) Intestinal phenotype comparison among wild type, klf4a-5mmMO1-, klf4a-5mmMO2, and klf4a-MO1 and klf4a-MO2-injected embryos at 96 hpf. The arrow indicates absence of intestinal folding and the arrowhead indicates no inflation of swim bladder. (D) Histological analysis of 96-hpf klf4a-MO1 and klf4a-MO2-injected morphants. I, intestine; L, liver.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-klf4 + MO2-klf4 AB + MO2-klf4
Knockdown Reagents:	MO1-klf4 MO2-klf4
Observed In:	eye intestine liver otic vesicle swim bladder tectal ventricle whole organism dorsal-ventral axis
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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