FIGURE

Fig. 3

ID

ZDB-FIG-110513-3

Publication

Li et al., 2011 - Regulation of endoderm formation and left-right asymmetry by miR-92 during early zebrafish development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

gata5 is a target of miR-92. (A) The GFP reporter fused to the 32UTR of gata5. Base pairing between miR-92 and two miRNA recognition elements (MREs) is shown. (B-G) Single-cell zebrafish embryos were injected with mRNAs encoding GFP reporters and fluorescence was monitored at 1 dpf. Embryos were injected with reporters containing either the full-length gata5 32UTR (full) or a construct in which both MREs were deleted (D12). MO refers to miR-92 MO. (H) Western blot of lysates from embryos injected as above were performed with antibodies against GFP or α-tubulin as a loading control. (I) Quantification of relative GFP expression. Error bars represent s.e.m. *, P<0.01, between indicated injection and control embryos (n>3); Student′s t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development