Fig. 2

Genotype and dystrophin expression of treated fish with seven candidate chemicals from the longer screen. (A) Genotyping of each of 40 fish treated with each of the seven potential candidate chemicals. Yellow, dystrophin-null affected fish (abnormal birefringence). Red, dystrophin-null unaffected fish (normal birefringence). Blue, heterozygous fish (normal birefringence). Light blue, WT fish (normal birefringence). In each of the seven chemically treated wells, some fish show normal birefringence despite genotyping results that show dystrophin-null fish. (B–J) Immunostaining with an anti-dystrophin antibody. (B) WT. (C) Dystrophin-null fish. (D–J) Dystrophin immunostaining of fish with restored muscle structure with each of the seven chemicals (chemicals 1–7 in Table 2). All restored fish with chemicals 1–7 showed no expression of dystrophin, similar to those of untreated dystrophin-null fish (C).