Fig. 4

Number of neuromasts assessed using live staining (FM1-43) in wild-type (A-C) at 72 hpf and with GFP visualisation in transgenic line (ET4) (D). The boxes symbolize the interquartile space of each distribution; the median is indicated with a horizontal line within the box, and dots indicate extreme values (5th/95th percentile). The number of individuals recorded for elaboration of the box plots is indicated with N = number of individuals. Numbers of neuromasts are shown in (A) ERβ₂ MO- or coMO-injected wild-type larvae (both MOs at 15 µM); (B) ERβ₂ MO-injected wild-type larvae (15 µM) co-injected with cRNA (300 ng); (C) ERβ₂ MO-injected wild-type larvae co-injected with p53MO (both at 15 µM). (D) ET4 larvae injected with ERβ₂ MO or coMO (both at 15 µM). One-way ANOVA following the Tukey test were performed to test the differences between blank (uninjected) and injected embryos and significances were indicated with * when p ≤ 0.05.