PUBLICATION
Estrogen receptor subtype beta2 is involved in neuromast development in zebrafish (Danio rerio) larvae
- Authors
- Froehlicher, M., Liedtke, A., Groh, K., Lopez-Schier, H., Neuhauss, S.C., Segner, H., and Eggen, R.I.
- ID
- ZDB-PUB-090319-13
- Date
- 2009
- Source
- Developmental Biology 330(1): 32-43 (Journal)
- Registered Authors
- Lopez-Schier, Hernan, Neuhauss, Stephan
- Keywords
- Estrogen Receptor, Neuromast, Zebrafish, Notch Signaling
- Datasets
- GEO:GSE13158, GEO:GSE13157, GEO:GSE13771
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Female
- Larva/metabolism
- Neurons, Afferent/metabolism*
- RNA, Messenger/metabolism
- Receptors, Estrogen/genetics
- Receptors, Estrogen/metabolism*
- Receptors, Notch/metabolism
- Zebrafish/embryology
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19289112 Full text @ Dev. Biol.
Citation
Froehlicher, M., Liedtke, A., Groh, K., Lopez-Schier, H., Neuhauss, S.C., Segner, H., and Eggen, R.I. (2009) Estrogen receptor subtype beta2 is involved in neuromast development in zebrafish (Danio rerio) larvae. Developmental Biology. 330(1):32-43.
Abstract
Estrogens are known to play a role in both reproductive and non-reproductive functions in mammals. Estrogens and their receptors are involved in the development of the central nervous system (brain development, neuronal survival and differentiation) as well as in the development of the peripheral nervous system (sensory-motor behaviors). In order to decipher possible functions of estrogens in early development of the zebrafish sensory system, we investigated the role of estrogen receptor beta(2) (ERbeta(2)) by using a morpholino (MO) approach blocking erbeta(2) RNA translation. We further investigated the development of lateral line organs by cell-specific labeling, which revealed a disrupted development of neuromasts in morphants. The supporting cells developed and migrated normally. Sensory hair cells, however, were absent in morphants' neuromasts. Microarray analysis and subsequent in situ hybridizations indicated an aberrant activation of the Notch signaling pathway in ERbeta(2) morphants. We conclude that signaling via ERbeta(2) is essential for hair cell development and may involve an interaction with the Notch signaling pathway during cell fate decision in the neuromast maturation process.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping