FIGURE

Fig. 6

ID

ZDB-FIG-110105-7

Publication

Seebald et al., 2011 - Zebrafish eve1 regulates the lateral and ventral fates of mesodermal progenitor cells at the onset of gastrulation

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Fig. 6

Whole-mount in situ hybridization using tbx6, floating head (flh) and chordin probes. A–L) Animal pole views of embryos at 6 hpf with dorsal to the right. A–D) tbx6 expression is normal in uninjected (A) (n = 28/28), dramatically reduced in eve/vent/vox morphant (B) (n = 65/86), substantially decreased in eve/ved/vent morphant (C) (n = 45/68), and not significantly affected in eve/vox/ved morphant (D) (n = 71/74). E–H) flh expression of uninjected (E) (n = 31/31), eve/vent/vox morphant (F) (n = 45/47), eve/ved/vent morphant (G) (n = 38/40), and eve/vox/ved morphant (H) (n = 32/35). I–L) chordin expression of uninjected (I) (n = 24/24), eve/vent/vox morphant (J) (n = 68/68), eve/ved/vent morphant (K) (n = 67/68), and eve/vox/ved morphant (L) (n = 28/28). Arrows indicate the ventral limit of chordin expression in wild-type (I) and eve/vox/ved morphant (L). Arrowheads indicate ventrally expanded chordin expression in eve/vent/vox (J) and eve/ved/vent (K) morphant embryos. Scale bar, 200 μm.

Expression Data

Genes:	chrd noto tbx16l
Fish:	AB AB + MO1-ved + MO1-vent + MO2-eve1 AB + MO1-ved + MO1-vox + MO2-eve1 AB + MO1-vent + MO1-vox + MO2-eve1
Knockdown Reagents:	MO1-ved MO1-vent MO1-vox MO2-eve1
Anatomical Terms:	presumptive dorsal mesoderm presumptive mesoderm presumptive mesoderm ventral region shield
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-ved + MO1-vent + MO2-eve1 AB + MO1-ved + MO1-vox + MO2-eve1 AB + MO1-vent + MO1-vox + MO2-eve1
Knockdown Reagents:	MO1-ved MO1-vent MO1-vox MO2-eve1
Observed In:	cellular response to BMP stimulus dorsal/ventral pattern formation presumptive mesoderm presumptive mesoderm ventral region
Stage:	Shield

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 349(1), Seebald, J.L., and Szeto, D.P., Zebrafish eve1 regulates the lateral and ventral fates of mesodermal progenitor cells at the onset of gastrulation, 78-89, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.