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Fig. 6 Whole-mount in situ hybridization using tbx6, floating head (flh) and chordin probes. A–L) Animal pole views of embryos at 6 hpf with dorsal to the right. A–D) tbx6 expression is normal in uninjected (A) (n = 28/28), dramatically reduced in eve/vent/vox morphant (B) (n = 65/86), substantially decreased in eve/ved/vent morphant (C) (n = 45/68), and not significantly affected in eve/vox/ved morphant (D) (n = 71/74). E–H) flh expression of uninjected (E) (n = 31/31), eve/vent/vox morphant (F) (n = 45/47), eve/ved/vent morphant (G) (n = 38/40), and eve/vox/ved morphant (H) (n = 32/35). I–L) chordin expression of uninjected (I) (n = 24/24), eve/vent/vox morphant (J) (n = 68/68), eve/ved/vent morphant (K) (n = 67/68), and eve/vox/ved morphant (L) (n = 28/28). Arrows indicate the ventral limit of chordin expression in wild-type (I) and eve/vox/ved morphant (L). Arrowheads indicate ventrally expanded chordin expression in eve/vent/vox (J) and eve/ved/vent (K) morphant embryos. Scale bar, 200 μm.
Reprinted from Developmental Biology, 349(1), Seebald, J.L., and Szeto, D.P., Zebrafish eve1 regulates the lateral and ventral fates of mesodermal progenitor cells at the onset of gastrulation, 78-89, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.