FIGURE

Fig. 3

ID

ZDB-FIG-101124-4

Publication

Yin et al., 2010 - Hand2 Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish

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Fig. 3

Hand2 Is Required for Gut Looping and LPM Migration

(A) foxa3 expression in wild-type and han^c99 mutants. Upper panel: the gut has looped to the left by 35 hpf in wild-types (22/22), but stays in the midline in han^c99 mutants (17/18). Dashed lines mark the midline. Lower panel: in wild-type by 48 hpf, the liver and pancreas are positioned on the left and right side of the gut, respectively (35/35). In han^c99 mutants, the liver (arrows) and/or pancreas (asterisks) is duplicated (12/12).

(B) At the 20-somite stage (19 hpf), spaw is expressed in the left LPM (arrows) in wild-types (70/70), but is absent from the LPM in most han^c99 mutants, or is bilateral in a few han^c99 mutants.

(C–G) Time course analysis shows that the Tg(hand2:EGFP)-expressing cells in han^c99 mutants fail to undergo asymmetric rearrangements. Ten han^c99 mutants were analyzed at each time point. Dashed lines outline the LPM.

(H) Diagram of LPM migration in han^c99 mutants. The gut is in yellow, the left LPM is in red, the right LPM is in blue, and the Tg(hand2:EGFP)-expressing cells are in green. The dark red line represents the apical side of the LPM epithelium. Arrows indicate the direction of migration of the Tg(hand2:EGFP)-expressing cells.

(A and B) Dorsal views, anterior to the top.

(C–H) Transverse sections, dorsal to the top.

L, left; Li, liver; NC, notochord; Pa, pancreas; R, right; So, somite. The scale bars represent 50 μm. See also Figure S2.

Expression Data

Genes:	EGFP foxa3 spaw
Fish:	WT hand2^c99/c99 hand2^c99/c99; pd24Tg
Anatomical Terms:	gut lateral plate mesoderm liver mesoderm lateral region pancreas
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hand2^c99/c99 hand2^c99/c99; pd24Tg
Observed In:	determination of intestine left/right asymmetry digestive tract development establishment of epithelial cell apical/basal polarity liver mesodermal cell migration pancreas
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 18(6), Yin, C., Kikuchi, K., Hochgreb, T., Poss, K.D., and Stainier, D.Y., Hand2 Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish, 973-984, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell