Fig. 3

Fig. 3 Hand2 Is Required for Gut Looping and LPM Migration

(A) foxa3 expression in wild-type and han^c99 mutants. Upper panel: the gut has looped to the left by 35 hpf in wild-types (22/22), but stays in the midline in han^c99 mutants (17/18). Dashed lines mark the midline. Lower panel: in wild-type by 48 hpf, the liver and pancreas are positioned on the left and right side of the gut, respectively (35/35). In han^c99 mutants, the liver (arrows) and/or pancreas (asterisks) is duplicated (12/12).

(B) At the 20-somite stage (19 hpf), spaw is expressed in the left LPM (arrows) in wild-types (70/70), but is absent from the LPM in most han^c99 mutants, or is bilateral in a few han^c99 mutants.

(C–G) Time course analysis shows that the Tg(hand2:EGFP)-expressing cells in han^c99 mutants fail to undergo asymmetric rearrangements. Ten han^c99 mutants were analyzed at each time point. Dashed lines outline the LPM.

(H) Diagram of LPM migration in han^c99 mutants. The gut is in yellow, the left LPM is in red, the right LPM is in blue, and the Tg(hand2:EGFP)-expressing cells are in green. The dark red line represents the apical side of the LPM epithelium. Arrows indicate the direction of migration of the Tg(hand2:EGFP)-expressing cells.

(A and B) Dorsal views, anterior to the top.

(C–H) Transverse sections, dorsal to the top.

L, left; Li, liver; NC, notochord; Pa, pancreas; R, right; So, somite. The scale bars represent 50 μm. See also Figure S2.