FIGURE

Fig. 1

ID

ZDB-FIG-101122-29

Publication

Soza-Ried et al., 2010 - Essential role of c-myb in definitive hematopoiesis is evolutionarily conserved

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Fig. 1

Phenotype of IP109 mutants. (A) Whole-mount RNA in situ hybridization of wild-type (^+/+) and homozygous IP109 mutant (^-/-) embryos with probes for ikaros (lateral views; Left) and rag1 (dorsal views; Right) at 5 d postfertilization (dpf). Note that ikaros is also expressed in neurons (arrows); this hybridization signal serves as an internal positive control for the hybridization process. In the hybridizations with rag1, a gh probe labels growth hormone-producing cells in the hypophysis (arrows) and serves as control for hybridization. The region of the thymus is encircled. No differences were seen between ^+/+ and ^+/m fish. Table S1 has probe details. (B) Macroscopic view of wild-type (Left) and mutant (Right) fish at 20 dpf. Note the smaller size and pale appearance of mutants. (Scale bar: 1 mm.) (C) Histological sections through the regions of the heart (A, atrium; V, ventricle) of heterozygous (Left) and homozygous mutant (Right) fish at 8 wk of age. Note the complete absence of erythrocytes in the mutant fish (Fig. S1). H&E staining. (Scale bars: 400 μm; Inset, 100 μm.)

Expression Data

Genes:	gh1 ikzf1 rag1
Fish:	WT myb^{t25127/t25127}
Anatomical Terms:	hypophysis cell neuron thymus hematopoietic multipotent progenitor cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myb^{t25127/t25127}
Observed In:	nucleate erythrocyte whole organism
Stage Range:	Days 14-20 to Days 45-89

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA