FIGURE

Fig. 4

ID

ZDB-FIG-101122-32

Publication

Soza-Ried et al., 2010 - Essential role of c-myb in definitive hematopoiesis is evolutionarily conserved

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Fig. 4

Failure of thymopoiesis in c-myb mutants. (A) Lack of thymus colonization in c-myb mutants transgenic for ikaros:eGFP. Still photographs were taken from Movies S9 and S10 at the indicated time points of the observation period (t₀ = 55 hpf), equivalent to <68 hpf. Note the cluster of green cells in the thymus (encircled), whereas the thymus of mutants lacks such cells. A progenitor cell approaching the thymus is seen at the bottom right corner of the image of wild-type fish. (B) No evidence for thymopoiesis in early larvae. WISH was done with probes indicated at 5 dpf; the thymic area (encircled) is shown. Note the presence of small numbers of cells expressing l-plastin in the c-myb mutants, a marker associated with the myelo-monocytic lineage. This most likely represents embryonic macrophages situated in the thymus (Movies S9 and S10).

Expression Data

Genes:	ccr9b EGFP gata3 lcp1 myb
Fish:	WT myb^{t25127/t25127} fr101Tg myb^{t25127/t25127}; fr101Tg
Anatomical Terms:	thymus hematopoietic multipotent progenitor cell thymus primordium hematopoietic multipotent progenitor cell
Stage Range:	Pec-fin to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA