FIGURE

Fig. 2

ID

ZDB-FIG-101119-7

Publication

Distel et al., 2009 - Optimized Gal4 genetics for permanent gene expression mapping in zebrafish

Other Figures

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Fig. 2

KalTA4GI enhancer trapping. (A) Schematic representation of the TK1xC enhancer trapping construct. (B) Examples of transgenic Gal4 enhancer trap lines at 50 hpf (C) kalTA4 mRNA expression (asterisk: olfactory bulb, arrow olfactory epithelium) in line olf:KalTA4. (D) Transactivation of lynGFP in Ulyn (10) injected olf:KalTA4 embryo. (E) KalTA4-mediated transactivation of GFP expression in offspring (26 hpf) from crosses of heterozygous olf:KalTA4 and 4xUAS-KGFPGI carriers. (F) Different expression patterns in F₁ embryos (50 hpf, lateral view) derived from the same P₀ founder fish. (G) Offspring of a rh3/5:KalTA4 homozygous carrier crossed to a wild-type fish. Note that all embryos display the characteristic mCherry fluorescence in rhombomeres 3 and 5. Embryos in B and F are counterstained with green Bodipy Ceramide.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA