ZFIN Image: Distel et al., 2009, Fig. 2

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Figure Caption

Fig. 2

KalTA4GI enhancer trapping. (A) Schematic representation of the TK1xC enhancer trapping construct. (B) Examples of transgenic Gal4 enhancer trap lines at 50 hpf (C) kalTA4 mRNA expression (asterisk: olfactory bulb, arrow olfactory epithelium) in line olf:KalTA4. (D) Transactivation of lynGFP in Ulyn (10) injected olf:KalTA4 embryo. (E) KalTA4-mediated transactivation of GFP expression in offspring (26 hpf) from crosses of heterozygous olf:KalTA4 and 4xUAS-KGFPGI carriers. (F) Different expression patterns in F₁ embryos (50 hpf, lateral view) derived from the same P₀ founder fish. (G) Offspring of a rh3/5:KalTA4 homozygous carrier crossed to a wild-type fish. Note that all embryos display the characteristic mCherry fluorescence in rhombomeres 3 and 5. Embryos in B and F are counterstained with green Bodipy Ceramide.

Acknowledgments

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