FIGURE

Fig. 7

ID

ZDB-FIG-101105-52

Publication

Butler et al., 2010 - Genetic and chemical modulation of spastin-dependent axon outgrowth in zebrafish embryos indicates a role for impaired microtubule dynamics in hereditary spastic paraplegia

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Fig. 7

Simultaneous knockdown of katna1 and spast enhances phenotypic severity. Immunostaining with znp-1 demonstrates that embryos co-injected with 0.3 pmol of spg4atg1 and 0.9 pmol of katna1aug1 morpholinos showed far more severe defects in spinal motor axon outgrowth (D) than embryos injected with either 0.3 pmol of spg4atg1 (B) or 0.9 pmol of katna1aug1 (C) alone. (A) Control embryo injected with 1.2 pmol of a control morpholino. (E) Quantification of mean spinal motor axon length in morpholino-injected embryos. Statistical significance was determined using ANOVA with Bonferroni’s multiple comparison test: ns, not significant; ** P<0.01; ***P<0.001. Scale bars: 50 μm.

Expression Data

Antibody:	znp-1
Fish:	WT WT + MO1-katna1 WT + MO1-spast WT + MO1-katna1 + MO1-spast
Knockdown Reagents:	MO1-katna1 MO1-spast
Anatomical Terms:	motor neuron axon spinal cord motor neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-katna1 WT + MO1-spast WT + MO1-katna1 + MO1-spast
Knockdown Reagents:	MO1-katna1 MO1-spast
Observed In:	axon extension motor neuron axon
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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