FIGURE

Fig. 2

ID

ZDB-FIG-101103-22

Publication

Vermot et al., 2009 - Reversing blood flows act through klf2a to ensure normal valvulogenesis in the developing heart

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Fig. 2

Decreased retrograde flow via lowered blood viscosity affects valve morphogenesis.

(A–D) Flow pattern at 48 hpf in (A) control and after (B) gata1, (C) gata2, and (D) gata1/2 knock down. gata2 inactivation leads to a dramatic decrease in the RFF, whereas gata1 and gata1/2 knock downs exhibit increased RFF compared to the control. (E–H) Confocal sections of the valve-forming region in (E) control, (F) gata1, (G) gata2, and (H) gata1/2 morphants. Only gata2 morphants at 96 hpf show valve dysgenesis. Scale bar indicates 50 µm. (I–K) Quantitative RT-PCR showing the expression level of several flow-responsive genes after gata1, gata2, or gata1/2 knock down. **p<0.01, ANOVA. (L) Percentage of embryos displaying valve malformation at 96 hpf (red bar), hematocrit level (yellow bar), and RFF (blue bar) observed in morphants and controls at 48 hpf. The proportions were significantly different at a 10% level of significance (α = 0.1). (M) Outline summarizing the experimental outcome of manipulating oscillatory flow by decreasing circulating blood cells. The color code for gene expression is the same as in (I).

Expression Data

Genes:	bmp4 edn1 klf2a notch1b nrg1
Fish:	WT WT + MO1-gata1a WT + MO1-gata2a WT + MO1-gata1a + MO1-gata2a
Knockdown Reagents:	MO1-gata1a MO1-gata2a
Anatomical Term:	heart
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gata1a WT + MO1-gata2a WT + MO1-gata1a + MO1-gata2a
Knockdown Reagents:	MO1-gata1a MO1-gata2a
Observed In:	atrioventricular valve atrioventricular valve formation blood blood circulating cell
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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