FIGURE

Fig. 3

ID

ZDB-FIG-100913-17

Publication

Gupta et al., 2010 - Microtubule actin crosslinking factor 1 regulates the Balbiani body and animal-vegetal polarity of the zebrafish oocyte

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Fig. 3

Germ plasm RNAs fail to localize to the cortex of mgn mutant oocytes.

vasa (A–D) and dazl (E–H) in situ hybridization to wild-type and mgn mutant oocytes. vasa mRNA is found in the Balbiani body in early stage I wild-type (heterozygous) oocytes (A, arrow), transiently localizes to the vegetal cortex in late stage I (A, arrowhead) and then localizes all around the cortex during stage II (B). In mgn mutant oocytes, vasa mRNA is found in the Balbiani body in early stage I oocytes (C, arrow) and is then found ectopically localized to the middle of the oocyte during stage II (D, arrow). In early stage I wild-type oocytes, dazl mRNA is found in the Balbiani body (E, arrow) and then localizes to the vegetal cortex during late stage I (E, arrowhead) and stage II (F). dazl mRNA localizes to the Balbiani body of early stage I mgn mutant oocytes (G, left oocyte, arrow) and is found ectopically localized to the middle of the oocyte during late stage I and stage II (G, right oocyte and H, respectively, arrow). Scale bars = 50 microns.

Expression Data

Genes:	dazl ddx4
Fish:	WT macf1a^p6cv/p6cv
Anatomical Terms:	oocyte stage I mitochondrial cloud oocyte stage II cell cortex oocyte stage II central region oocyte stage II cytoplasm
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	macf1a^p6cv/p6cv
Observed In:	oocyte animal/vegetal axis specification oocyte differentiation oocyte stage II central region oocyte stage II cytoplasm
Stage:	Adult

Phenotype Detail

Acknowledgments

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