FIGURE

Fig. S4

ID

ZDB-FIG-100603-37

Publication

Piloto et al., 2010 - Ovo1 links Wnt signaling with N-cadherin localization during neural crest migration

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Fig. S4

Identification of pac^par2.10 (ncad^b>⁺^/b>^/^b>^-) mutants. (A) Schematic of the different ncad mutant isoforms. The wild-type product is 266 bp; mutants contain either a smaller 143 bp form or larger isoforms with as many as 50 additional nucleotides. (B,C) RT-PCR for ncad in pac^par2.10 heterozygous embryos injected with Ovo1 MO (B, all lanes; C, lanes 6-8) or with Rab11fip2 RNA (C, lanes 3-5). All embryos with ectopic dorsal NC cells (B, lanes 7-11; C, lanes 3,4,6,7) are heterozygous for pac^par2.10, whereas embryos whose NC cells migrated normally do not exhibit alternative spliced forms of ncad (B, lanes 2-6; C, lanes 5,8). Uninjected pac^par2.10 heterozygous control (C, lane 2).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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