Fig. S5

Morpholino Knockdown of erm, pea3 and etv5 in Zebrafish
For each gene, the primary transcript is schematized; morpholino target sites are marked above with a red bar and the position of primers used to determine the resulting splicing pattern is indicated below. The DNA binding domain of erm, pea3 and etv5 is located in the 3′ region of each transcript and is spread across 3 exons as indicated in green. The predicted protein following MO injection is presented below: a non-specific amino acid fusion is indicated in red. erm morpholino was directed against the splice acceptor site upstream of exon 11 and results in a 79bp insertion of the entire intron 10. A stop codon is observed 3 amino acids into the predicted fusion protein, terminating the protein prior to critical elements required for DNA binding based on homology with other Ets-DNA binding transcription factors. pea3 morpholino was directed against the splice acceptor site upstream of exon 10 and results in a 173bp deletion, removing exon 9. This deletion puts the resultant protein out of frame creating an in-frame stop codon 15 amino acids into the predicted fusion protein. etv5 morpholino was directed against the splice acceptor site upstream of exon 7 and results in a 94bp insertion of the intron 6 resulting in translational termination at a stop codon 35 amino acids into the predicted fusion transcript.