FIGURE

Fig. 3

ID

ZDB-FIG-100504-66

Publication

Mao et al., 2009 - Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb

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Fig. 3

Ectopic Activation of Shh and Shh Targets Results from an Inhibition of Etv-Dependent Regulation

(A) Shh expression at different stages of limb development in R26-EtvEnR and Prx1-Cre;R26-EtvEnR embryos.

(B) Expression of Gli1, Hand2, Alx4, and Gremlin transcripts in the limb buds of R26-EtvEnR and Prx1-Cre;R26-EtvEnR embryos at E10.5.

(C) Dorsal view of Shh expression in the zebrafish pectoral fin bud 48 hr postfertilization (upper); a higher-magnification view (lower). The scale bars represent 0.02 mm.

(D) Quantitative real-time PCR analysis of Gli1 mRNA levels after culturing of anterior limb bud cells for 24 hr in the presence or absence of Fgf8.

Expression Data

Gene:	shha
Fish:	WT WT + MO1-etv5a + MO2-etv4 + MO2-etv5b
Knockdown Reagents:	MO1-etv5a MO2-etv4 MO2-etv5b
Anatomical Term:	pectoral fin bud
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-etv5a + MO2-etv4 + MO2-etv5b
Knockdown Reagents:	MO1-etv5a MO2-etv4 MO2-etv5b
Observed In:	pectoral fin bud
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 16(4), Mao, J., McGlinn, E., Huang, P., Tabin, C.J., and McMahon, A.P., Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb, 600-606, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell