PUBLICATION
Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb
- Authors
- Mao, J., McGlinn, E., Huang, P., Tabin, C.J., and McMahon, A.P.
- ID
- ZDB-PUB-100406-1
- Date
- 2009
- Source
- Developmental Cell 16(4): 600-606 (Journal)
- Registered Authors
- Huang, Peng, McMahon, Andrew
- Keywords
- DEVBIO
- MeSH Terms
-
- Repressor Proteins
- Mesoderm/cytology
- Mesoderm/embryology
- Mesoderm/metabolism
- Mice
- Proto-Oncogene Proteins c-ets/metabolism*
- Transcription Factors/metabolism*
- Paired Box Transcription Factors/metabolism
- Cells, Cultured
- Hedgehog Proteins/metabolism*
- Cell Polarity
- DNA-Binding Proteins/metabolism*
- Fibroblast Growth Factor 8/metabolism*
- Ectoderm/cytology
- Ectoderm/embryology
- Ectoderm/metabolism
- Integrases/metabolism
- Polydactyly/pathology
- Vertebrates/embryology*
- Animals
- Extremities/embryology*
- Body Patterning*
- Chick Embryo
- PubMed
- 19386268 Full text @ Dev. Cell
Citation
Mao, J., McGlinn, E., Huang, P., Tabin, C.J., and McMahon, A.P. (2009) Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb. Developmental Cell. 16(4):600-606.
Abstract
Crosstalk between the fibroblast growth factor (FGF) and Sonic Hedgehog (Shh) pathways is critical for proper patterning and growth of the developing limb bud. Here, we show that FGF-dependent activation of the ETS transcription factors Etv4 and Etv5 contributes to proximal-distal limb outgrowth. Surprisingly, blockage of Etv activity in early distal mesenchyme also resulted in ectopic, anterior expansion of Shh, leading to a polydactylous phenotype. These data indicate an unexpected function for an FGF/Etv pathway in anterior-posterior patterning. FGF activity in the limb is not only responsible for maintaining posterior-specific Shh expression, but it also acts via Etvs to prevent inappropriate anterior expansion of Shh. This study identifies another level of genetic interaction between the orthogonal axes during limb development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping