FIGURE

Fig. 3

ID

ZDB-FIG-100323-5

Publication

Chablais et al., 2010 - IGF signaling between blastema and wound epidermis is required for fin regeneration

Other Figures

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Fig. 3

Morpholino-mediated igf2b and igf1rab knockdowns impair fin regeneration. (A-E) Fins microinjected into their dorsal half with control (A), igf2b (B), igf1ra + igf1rb (C), igf1ra (D) and igf1rb (E) MOs. The remaining non-injected half of the fin served as an internal control to assess the normal growth rate. The left panels show fins shortly after injection and the right panels show the same fins at 48 hpi. The control MO does not affect the size of the outgrowth relative to the uninjected side (A). The specific MOs impair regeneration, leading to an asymmetric shape of the regenerate (B-E). (F) Bar chart comparing the surface of regenerates that regrew within 24 and 48 hours after MO injection in relation to the uninjected fin regenerate. The injected:uninjected regenerate surface is significantly reduced by delivery of specific MOs. n=10 fins per group; *P<0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-igf1ra WT + MO1-igf1rb WT + MO4-igf2b WT + MO1-igf1ra + MO1-igf1rb
Condition:	physical alteration: anatomical structure
Knockdown Reagents:	MO1-igf1ra MO1-igf1rb MO4-igf2b
Observed In:	fin regeneration
Stage:	Adult

Phenotype Detail

Acknowledgments

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