FIGURE

Fig. 4

ID

ZDB-FIG-100323-6

Publication

Chablais et al., 2010 - IGF signaling between blastema and wound epidermis is required for fin regeneration

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Fig. 4

Igf1r signaling is required for the establishment of the wound epidermis. (A-C) Longitudinal sections of fins at 72 hpa stained with Hematoxylin and Eosin. The dashed line marks the amputation plane. In control fin regenerates, the basal epithelium consists of ordered linear cuboidal epithelial cells in the proximal part and columnar epithelial cells at the apical end. The blastema is also indicated by an arrow (A). Fins treated with either NVP-AEW541 (B) or NVP-ADW742 (C) lack a distinctive basal layer of the wound epidermis and the blastema. (D-F) Longitudinal sections of fins at 24 hpa stained with active-Caspase-3 antibody (green) and a nuclear marker DAPI (blue). Control fins display only individual active-Caspase-3-positive cells (D). Exposure to either NVP-AEW541 (E) or NVP-ADW742 (F) markedly enhances cell apoptosis specifically in the wound epidermis. (G) Quantification of active-Caspase-3 in control and inhibitor-treated fins at 24 hpa. n=6; *P<0.01. bl., blastema; col. ep., columnar epithelial cells; cub. ep., cuboidal epithelial cells; we, wound epidermis. Scale bars: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: pharmaceutical, physical alteration: anatomical structure
Observed In:	basal regeneration epithelium blastema fin regeneration regeneration epithelium
Stage:	Adult

Phenotype Detail

Acknowledgments

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