Fig. 5

Analysis of sensory ganglion formation in hmx2/hmx3 morphant embryos. Anterior is to the left and the orientation as indicated. (A–D) hmx3 expression in control (A, C) and morphant (B, D) embryos, with positions of the transverse sections (C′, D′) as indicated. The black arrowhead indicates hmx3-expressing cells anterior to the otic vesicle in the control, and the black arrow indicates the absence of this cell population in morphants. The white arrowhead indicates the ventral floor in control embryos and the white arrow indicates hmx3-expressing cells retained at the ventral floor in morphants. (E, F) Confocal images of a single stack, showing expression of hmx3 (red), neuroD (green) and anti-Hu (blue) in the otic vesicle of wild-type embryos at 28 hpf (E) and 48 hpf (F). The green and blue lines in XY plane indicate the position of the ZX and ZY sections, respectively. hmx3-expressing cells (red) are only present in gAV at 28 hpf and gAV/VII at 48 hpf. (G–N) Triple detection of smad1 (red), hmx3 (green), and anti-HNK1 (blue) in wild-type (G–K, M) or hmx2/3 morphant (L, N) embryos at the indicated stages. The pictures show projections of confocal stacks through either the dorsal (G, I, K, L) or ventral (H, J, M, N) part of the otic vesicle. (O–Q) Detection of Hu protein in control (O) and hmx2/3 morphant (P, Q) embryos. Dotted lines outline the structure indicated in the panel. Abbreviations: gAD, dorsal branch of the anterior lateral line ganglion; gAV/VII, ventral branch of the anterior lateral line ganglion (gAV) fused with facial sensory ganglion (gVII); SAg, statoacoustic ganglion; Tg, trigeminal ganglion. Scale bar: 50 μm except 25 μm in panel D′.