FIGURE

Fig. 3

ID

ZDB-FIG-100319-25

Publication

Feng et al., 2010 - Pivotal role of hmx2 and hmx3 in zebrafish inner ear and lateral line development

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Fig. 3

Reduced number of hair cells and progressive fusion of two maculae in hmx2/hmx3 morphants. Anterior to the left, dorsal view in panels A–L and lateral view in panels M–R. (A–F) Hair cells are labeled with antibodies for S-100 (red) and acetylated-tubulin (green) at the stages indicated showing progressive fusion of the anterior (utricular) maculae with the posterior (saccular) maculae at the medial wall in the morphants. (G–N) In situ hybridization with Na,K-ATPase β2b probe to reveal mature hair cells, showing a reduced number in the anterior maculae (amac) and fusion with the posterior maculae (pmac). (O–R) Rhodamine–phalloidin staining of the F-actin of the hair bundles in control (O, Q) and morphants (P, R). (S) Illustration of control amac and pmac from 9 ears. (T) Illustration of fused maculae in morphants from 8 ears. Arrowheads indicate hair cells. Scale bars: (A–F) 50 μm, (G–R) 25 μm. Abbreviations: AM, anterior maculae; PM, posterior maculae; M, maculae fusion.

Expression Data

Gene:	atp1b2b
Antibody:	Ab1-s100
Fish:	AB AB + MO1-hmx2 + MO2-hmx3a
Knockdown Reagents:	MO1-hmx2 MO2-hmx3a
Anatomical Terms:	anterior macula macula posterior macula
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-hmx2 + MO2-hmx3a
Knockdown Reagents:	MO1-hmx2 MO2-hmx3a
Observed In:	anterior macula hair cell anterior macula macula neuromast hair cell posterior macula tether cell
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 339(2), Feng, Y., and Xu, Q., Pivotal role of hmx2 and hmx3 in zebrafish inner ear and lateral line development, 507-518, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.