FIGURE

Fig. 3

ID

ZDB-FIG-100302-45

Publication

Zhang et al., 2010 - Establishment of a neuroepithelial barrier by Claudin5a is essential for zebrafish brain ventricular lumen expansion

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Fig. 3

Loss of Claudin5a affects expansion of the brain ventricular lumen at 20 hpf. (A–E) Shown are confocal microscopic Z-scans of in vivo sodium green indicator-labeled embryos. The ventricular lumen is indicated by false-coloring (orange). Indicated are ventricles of the midbrain (MBV) and hindbrain (HBV). (F) Quantifications of ventricular volume were generated from detailed 3D-reconstructions of confocal Z-scan sections for a total of 4–5 embryos per sample group by using Volocity software (unit size is [x10⁶ μm³]). Data represent mean ± SEM, n ≥ 4, *, P = 0.016 in all three cases. (Scale bar: 100 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-cldn5a AB + MO2-cldn5a + MO4-tp53
Knockdown Reagents:	MO2-cldn5a MO4-tp53
Observed In:	fourth ventricle tectal ventricle ventricular system ventricular system development
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ Proc. Natl. Acad. Sci. USA