Fig. S3

(A-D) Representative images for the AChR prepattern rescue in 21-somite Tg(hsp70l:unplugged SV1-myc); unplugged^br307/br307 embryos after HS treatments. Embryos were stained for motor neurons (znp-1, green) and AChRs (α-BTX, red). Segments 11-15 were imaged in each embryo. (E-H) Tg(hsp70l:unplugged SV1-myc); unplugged^br307/br307 embryos were heat-shocked for 40 minutes at the 20-somite stage and examined at the 21-somite stage for motor neurons and AChRs. AChR prepatterning was not well rescued in segments 11-15 (E and G). Posterior segments 16-20 from the same embryo display better rescue of the prepatterning (F and H). (G and H) Enlarged images of the highlighted segments in E and F. Arrow in (H) indicate the large wildtype-like prepatterned clusters. Arrowheads in (G) mark the small punctate clusters. Scale bars: 20 μM. (I-L) Rescue of neural synapses at 48hpf by Tg(hsp70l:unplugged FL-myc). Embryos were heat-shocked for 40 minutes at 48hpf, fixed at 51hpf and stained for motor axons (znp-1, green) and AChR clusters (α-BTX, red). (I and J) Neural synapses induced by heat shock treatment of Tg(hsp70l:unplugged FL-myc); unplugged^br307/br307 embryos persisted for 3 hours following the heat shock. (K and L) No neural synapses were induced in unplugged^br307/br307 embryos lacking the transgene. Arrows in I and K point to rescued and non-rescued synapses respectively with insets showing enlarged views.